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Phlebotomy

Blood Specimen Tubes

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Blood for diagnostic investigations can be collected either from the peripheral vein or from the capillaries. Venous blood is preferred for hematology, biochemistry, serology & immunology investigations but capillary blood can be nearly as accurate if a free flow of blood is obtained and there is no dilution error due to tissue fluids.

Site Selection

Blood is usually collected from veins, capillaries and arteries. Venous blood is collected from anticubital vein most commonly. Blood is also collected from the vein of arms, dorsum of hands. Long cephalic vein is also used where non-availability of the former veins. Infant’s femoral vein is used for collection of blood. In newborn children umbilical vein and scalp vein are commonly used for puncture.

For collection of capillary blood, the site is selected usually the finger-tips or ear lobe in adult and the great toe or heel in infants


Order of Drawing Blood

Blood Draw Order Tube Order Description
1 Color Varies Blood Cultures
2 Light Blue Sodium Citrate
3 Red Clot Activator
4 Gold SST
5 Light Green Lithium Heparin
6 Dark Green Sodium Heparin
7 Lavender EDTA
8 Gray Sodium Fluoride


Order of Draw Acronym

Sometimes the trick to remembering a lot of informationis to create a mnemonic acronym. This helps break the information down into easy to remember chunks, with just enough info in each chunk to help you remember the bigger picture. Mnemonic acronyms are not set in stone, and you can create your own acronym to help you easily remember the order of blood draw. An example of this could be "Bunnies Love Roaming Gardens, Looking for Delicious Leafy Greens.

Bunnies Blood Culture
Love Light Blue
Roaming Red
Gardens Gold
Looking (For) Light Green
Delicious Dark Green
Leafy Lavender
Greens Gray
Gardens Gold
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